Enhanced insulin-receptor tyrosine kinase activity associated with chromosomal translocation (1;19) in a pre-B-cell leukemia line

Int J Cancer. 1992 Feb 1;50(3):500-4. doi: 10.1002/ijc.2910500328.


The gene for the insulin receptor has been assigned to chromosome 19 near the breakpoint of the translocation t(1;19) which occurs in 25% of pre-B-cell leukemias. Insulin receptors in a pre-B-cell leukemia cell line (ACV) with t(1;19) were found to have 2-fold higher affinity for insulin, 5-fold higher basal and insulin-stimulated beta sub-unit autophosphorylation, and 2-fold higher basal and 4-fold higher insulin-stimulated beta sub-unit kinase activity on the synthetic peptide poly(Glu,Tyr), compared to receptors in a B-cell line (ADD) with normal karyotype from the same patient. ACV cells had a novel 13-kb receptor mRNA species and expressed a DNA polymorphism localized to the tyrosine kinase domain of the receptor gene. These findings suggest that t(1;19) in the ACV cell may result in rearrangement of the insulin receptor gene and translation of a receptor with enhanced tyrosine kinase activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Northern
  • Blotting, Southern
  • Chromosomes, Human, Pair 1
  • Chromosomes, Human, Pair 19
  • DNA, Neoplasm / genetics
  • Gene Expression
  • Humans
  • In Vitro Techniques
  • Insulin / metabolism
  • Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / genetics*
  • Protein-Tyrosine Kinases / genetics*
  • Protein-Tyrosine Kinases / metabolism
  • RNA, Messenger / genetics
  • RNA, Neoplasm / genetics
  • Receptor, Insulin / metabolism
  • Translocation, Genetic
  • Tumor Cells, Cultured


  • DNA, Neoplasm
  • Insulin
  • RNA, Messenger
  • RNA, Neoplasm
  • Protein-Tyrosine Kinases
  • Receptor, Insulin