Cloning and nucleotide sequence of the Escherichia coli K-12 ppsA gene, encoding PEP synthase

Mol Gen Genet. 1992 Jan;231(2):332-6. doi: 10.1007/BF00279808.


We have cloned and sequenced the Escherichia coli K-12 ppsA gene. The ppsA gene codes for PEP synthase, which converts pyruvate into phosphoenolpyruvate (PEP), an essential step in gluconeogenesis when pyruvate or lactate are used as a carbon source. The open reading frame consists of 792 amino acids and shows homology with other phosphohistidine-containing enzymes that catalyze the conversion between pyruvate and PEP. These enzymes include pyruvate, orthophosphate dikinases from plants and Bacteroides symbiosus and Enzyme I of the bacterial PEP:carbohydrate phosphotransferase system.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Cloning, Molecular
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Genes, Bacterial*
  • Histidine / analogs & derivatives
  • Histidine / genetics
  • Molecular Sequence Data
  • Pyruvate, Orthophosphate Dikinase / chemistry
  • Pyruvate, Orthophosphate Dikinase / genetics*
  • Sequence Alignment
  • Sequence Homology, Nucleic Acid


  • Bacterial Proteins
  • Histidine
  • Pyruvate, Orthophosphate Dikinase
  • phosphohistidine

Associated data

  • GENBANK/M69116
  • GENBANK/X59381