Analysis of tyrosine kinase mRNAs including four FGF receptor mRNAs expressed in MCF-7 breast-cancer cells

Int J Cancer. 1992 Feb 20;50(4):598-603. doi: 10.1002/ijc.2910500419.

Abstract

The MCF-7 cell line is a hormone-responsive human breast-cancer cell line, which has been extensively used in studies of estrogen regulation of cell growth. These studies have indicated that the growth stimulation of the MCF-7 cells by estrogens may be effected by an autocrine mechanism involving several growth factors, such as EGF, TGF alpha and IGF-I and their receptors. We have amplified and cloned tyrosine-kinase-related sequences from the MCF-7 cell mRNA using the polymerase chain reaction and characterized the partial cDNAs obtained by nucleic acid sequencing. Nine tyrosine kinase cDNAs and one serine/threonine kinase cDNA were identified among the amplified sequences. Four different tyrosine kinase genes encoding receptors for fibroblast growth factors (FGFs) were found to be expressed by the MCF-7 cells. In addition, differences were observed in the expression of these members of FGF receptor family in different breast-cancer cells. A putative tyrosine-kinase receptor and a novel serine/threonine kinase were preferentially expressed in estrogen-responsive tumor cell lines. However, no estrogen-dependent regulation of any of the novel tyrosine-kinase receptor mRNAs was found in any of the cell lines including the MCF-7 or ZR-75-I cells, where the expression of the neu proto-oncogene mRNA was decreased during estrogen treatment. The expression of several FGF receptors by breast-cancer cells suggests that FGFs may be involved in their growth regulation and tumorigenesis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Breast Neoplasms / genetics*
  • Cloning, Molecular
  • Gene Expression
  • Gene Expression Regulation, Neoplastic / drug effects
  • Humans
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides / chemistry
  • Polymerase Chain Reaction
  • Protein Kinase C / genetics
  • Protein Kinases / genetics
  • Protein-Serine-Threonine Kinases
  • Protein-Tyrosine Kinases / genetics*
  • RNA, Messenger / genetics
  • RNA, Neoplasm / genetics
  • Receptors, Cell Surface / genetics*
  • Receptors, Fibroblast Growth Factor
  • Sequence Alignment
  • Steroids / pharmacology
  • Tumor Cells, Cultured

Substances

  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • RNA, Neoplasm
  • Receptors, Cell Surface
  • Receptors, Fibroblast Growth Factor
  • Steroids
  • Protein Kinases
  • Protein-Tyrosine Kinases
  • Protein-Serine-Threonine Kinases
  • Protein Kinase C