Immunohistochemical demonstration of the Epstein-Barr virus-encoded latent membrane protein in paraffin sections of Hodgkin's disease

J Pathol. 1992 Jan;166(1):1-5. doi: 10.1002/path.1711660102.


Paraffin sections from 46 cases of Hodgkin's disease were examined for the presence of the Epstein-Barr virus (EBV)-encoded latent membrane protein (LMP) using a sensitive (double layer alkaline phosphatase-anti-alkaline phosphatase) immunohistochemical method. LMP was detected in 22 cases, the majority of positive cases being of nodular sclerosis (12/24), mixed cellularity (6/7), and lymphocyte depletion (3/3) subtypes. Only one of 12 cases of lymphocyte predominant disease was positive. In all cases, reactivity was confined to Hodgkin's and Reed-Sternberg cells. These results provide further evidence for an association between EBV and Hodgkin's disease and indicate that LMP may be readily detected in archival material.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Herpesvirus 4, Human / isolation & purification*
  • Hodgkin Disease / microbiology*
  • Humans
  • Immunoenzyme Techniques
  • Lymph Nodes / microbiology
  • Membrane Proteins / analysis*
  • Paraffin Embedding
  • Viral Proteins / analysis*


  • Membrane Proteins
  • Viral Proteins