Induction of Mn-superoxide dismutase by tumor necrosis factor, interleukin-1 and interleukin-6 in human hepatoma cells

Biochem Biophys Res Commun. 1992 Feb 14;182(3):1100-7. doi: 10.1016/0006-291x(92)91845-h.


Effects of Tumor Necrosis Factor (TNF), Interleukin-1 (IL-1), Interleukin-6 (IL-6) and Interferon-gamma (IFN-gamma) on the expression of Mn-superoxide dismutase (Mn-SOD) protein were investigated in human hepatoma cells, Hu-H1, which revealed resistance to the cytotoxicity of TNF and IL-1. Both TNF and IL-1 enhanced the Mn-SOD production to the level of 30- to 40-fold. IL-6 also increased the enzyme protein to 2- to 3-fold of the basal level without any cell proliferative effect. A specific antibody against IL-6 almost completely inhibited the induction of Mn-SOD. IL-6, as well as TNF and IL-1, appears to play some role in the Mn-SOD protein expression in human hepatoma cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies
  • Carcinoma, Hepatocellular
  • Cell Division / drug effects
  • Cell Line
  • DNA Replication / drug effects
  • Enzyme Induction
  • Humans
  • Interferon-gamma / pharmacology
  • Interleukin-1 / pharmacology*
  • Interleukin-6 / pharmacology*
  • Kinetics
  • Liver Neoplasms
  • Recombinant Proteins / pharmacology
  • Superoxide Dismutase / biosynthesis*
  • Thymidine / metabolism
  • Tumor Necrosis Factor-alpha / pharmacology*


  • Antibodies
  • Interleukin-1
  • Interleukin-6
  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha
  • Interferon-gamma
  • Superoxide Dismutase
  • Thymidine