A Comparative Study of Hemocytes From Six Different Snails: Morphology and Functional Aspects

J Invertebr Pathol. 1992 Jan;59(1):24-32. doi: 10.1016/0022-2011(92)90107-f.

Abstract

Hemocytes taken from six different gastropod snails, Achatina achatina, A. fulica, Biomphalaria glabrata, Bulinus natalensis, Helix aspersa, and Lymnaea stagnalis, were compared for morphology, peroxidase activity, and, using methods developed for L. stagnalis, the ability to generate reactive oxygen inermediates upon phagocytic stimulation. Numbers of hemocytes per milliliter hemolymph and hemocytes' microscopical morphology showed some variation among the snail species. Peroxidase activity was demonstrated in all snail hemocytes except in those of B. glabrata and A. fulica. Hemocytes of all species generated superoxide upon phagocytic stimulation with zymosan (tested by superoxide dismutase-inhibitable reduction of nitroblue tetrazolium). When tested, hemocytes of A. achatina and of A. fulica displayed luminol-dependent chemiluminescence activity.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Count
  • Hemocytes / cytology*
  • Hemocytes / enzymology
  • Hemocytes / physiology
  • Peroxidase / analysis
  • Snails / cytology*
  • Superoxides / metabolism

Substances

  • Superoxides
  • Peroxidase