An ESR-CT imaging of the head of a living rat receiving an administration of a nitroxide radical

Magn Reson Imaging. 1992;10(1):109-14. doi: 10.1016/0730-725x(92)90379-e.

Abstract

Three-dimensional ESR imaging of a living rat has been performed by an L-band ESR system, which is composed of an L-band ESR spectrometer, a field gradient coil, and a data processor. The imaging was carried out by Lauterbur's method. A nitroxide, 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl (Carbamoyl-PROXYL), was used as an imaging agent in saline solution at a concentration of 0.2 M and administered intraperitoneally to obtain a constant concentration in the head for about an hour. It took about 40 min to obtain one set of ESR-CT images. The cross-sectional images were made, both as coronal and horizontal images. In the images of the rat head the nitroxide-rich region was clearly distinguished from the deficient region. The nitroxide-deficient areas corresponded well to the brain of the rat.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / anatomy & histology*
  • Cyclic N-Oxides*
  • Diagnostic Imaging / methods*
  • Electron Spin Resonance Spectroscopy*
  • Image Processing, Computer-Assisted*
  • Male
  • Rats
  • Rats, Inbred Strains
  • Spin Labels*

Substances

  • Cyclic N-Oxides
  • Spin Labels
  • 3-carbamoyl-2,2,5,5-tetramethyl-3-pyrroline-1-yloxyl