Demonstration of retrotransposition of the Tf1 element in fission yeast

EMBO J. 1992 Mar;11(3):1145-53.

Abstract

Tf1, a retrotransposon from fission yeast, has LTRs and coding sequences resembling the protease, reverse transcriptase and integrase domains of retroviral pol genes. A unique aspect of Tf1 is that it contains a single open reading frame whereas other retroviruses and retrotransposons usually possess two or more open reading frames. To determine whether Tf1 can transpose, we overproduced Tf1 transcripts encoded by a plasmid copy of the element marked with a neo gene. Approximately 0.1-4.0% of the cell population acquired chromosomally inherited resistance to G418. DNA blot analysis demonstrated that such strains had acquired both Tf1 and neo specific sequences within a restriction fragment of the same size; the size of this restriction fragment varied between different isolates. Structural analysis of the cloned DNA flanking the Tf1-neo element of two transposition candidates with the same regions in the parent strain showed that the ability to grow on G418 was due to transposition of Tf1-neo and not other types of recombination events.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Blotting, Southern
  • DNA Probes
  • DNA Transposable Elements*
  • DNA, Fungal / genetics
  • Genes, Fungal
  • Molecular Sequence Data
  • Plasmids
  • Promoter Regions, Genetic
  • RNA, Fungal / genetics
  • Repetitive Sequences, Nucleic Acid
  • Saccharomyces cerevisiae / genetics
  • Schizosaccharomyces / genetics*
  • Species Specificity
  • Temperature
  • Transcription, Genetic

Substances

  • DNA Probes
  • DNA Transposable Elements
  • DNA, Fungal
  • RNA, Fungal

Associated data

  • GENBANK/L12698
  • GENBANK/L12699
  • GENBANK/L12700
  • GENBANK/L12701
  • GENBANK/L12702
  • GENBANK/L12703
  • GENBANK/S87961
  • GENBANK/S87965
  • GENBANK/S87967
  • GENBANK/S87972