Bacillus marisflavi sp. nov. and Bacillus aquimaris sp. nov., isolated from sea water of a tidal flat of the Yellow Sea in Korea

Int J Syst Evol Microbiol. 2003 Sep;53(Pt 5):1297-1303. doi: 10.1099/ijs.0.02365-0.


Two Gram-positive or Gram-variable, endospore-forming, moderately halophilic rods (strains TF-11(T) and TF-12(T)) were isolated from a tidal flat of the Yellow Sea in Korea and were subjected to a polyphasic taxonomic study. Strains TF-11(T) and TF-12(T) had cell-wall peptidoglycan based on meso-diaminopimelic acid. The predominant menaquinone found in the two strains was MK-7. The cellular fatty acid profiles of both strains contained large amounts of branched and saturated fatty acids. The major fatty acids were anteiso-C(15 : 0) and iso-C(15 : 0). The DNA G+C contents of strains TF-11(T) and TF-12(T) were respectively 49 and 38 mol%. Phylogenetic analysis based on 16S rDNA sequences showed that strains TF-11(T) and TF-12(T) fell within the radiation of the cluster comprising Bacillus species. The level of 16S rDNA sequence similarity between strains TF-11(T) and TF-12(T) was 98.3 %. Strains TF-11(T) and TF-12(T) exhibited levels of 16S rDNA sequence similarity of less than 96.0 and 96.3 %, respectively, to Bacillus species. The mean level of DNA-DNA relatedness between the two strains was approximately 7 %. On the basis of phenotypic and phylogenetic data and genomic distinctiveness, strains TF-11(T) and TF-12(T) should be placed in the genus Bacillus as two distinct novel species, for which the names Bacillus marisflavi sp. nov. (type strain TF-11(T)=KCCM 41588(T)=JCM 11544(T)) and Bacillus aquimaris sp. nov. (type strain TF-12(T)=KCCM 41589(T)=JCM 11545(T)) are proposed.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus / classification
  • Bacillus / genetics
  • Bacillus / isolation & purification*
  • Bacillus / metabolism
  • Base Composition
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / genetics
  • Fatty Acids / analysis
  • Korea
  • Microscopy, Electron
  • Molecular Sequence Data
  • Phenotype
  • Phylogeny
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • Seawater / microbiology
  • Species Specificity


  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • RNA, Bacterial
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/AF483624
  • GENBANK/AF483625