Differential expression of myogenic regulatory genes, MyoD1 and myogenin, in human rhabdomyosarcoma sublines

Int J Cancer. 1992 Apr 1;50(6):977-83. doi: 10.1002/ijc.2910500626.


A cell line (SCMC-MM-1) was established from a human abdominal tumor that was initially diagnosed as a malignant mesenchymoma by histological, immunohistochemical and clinical criteria. The cell line was composed of 2 morphologically and immunohistochemically distinct cell types, one with a small polygonal phenotype (P-type), characterized by the immunostaining of vimentin and the presence of a few electron-microscopically visible organelles, and the other with a giant tubular phenotype (T-type), characterized by the immunostaining of desmin, alpha-sarcomeric actin and skeletal-muscle myosin, and the presence of thick and thin myofilaments and Z-line materials. The parental cell line was cloned into 2 sublines, a P-type clone (SCMC-MM-1-19P) and a T-type clone (SCMC-MM-1-1T), which shared both 2q37 and 11p15 translocations, the characteristic chromosomal aberrations for rhabdomyosarcoma, with the parental SCMC-MM-1 cell line. Northern-blot analyses of the myogenic regulatory genes, including MyoD1 and myogenin, demonstrated the expression of MyoD1 in both of these sublines. Myogenin was very weakly expressed in the SCMC-MM-1-19P subline, but strongly expressed in the SCMC-MM-1-1T subline. Chromosomal and myogenic-regulatory-gene analyses revealed that both of these sublines were rhabdomyosarcoma cell lines. Furthermore, the regulatory-gene analyses indicated that these 2 sublines represented 2 distinct differentiation stages of myoblasts, and that MyoD1 and myogenin could serve as the lineage marker and the differentiation marker, respectively, of human rhabdomyosarcoma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Northern
  • Chromosome Banding
  • Clone Cells
  • Fluorescent Antibody Technique
  • Genes, Regulator*
  • Genetic Markers
  • Humans
  • Karyotyping
  • Microscopy, Electron
  • Muscle Proteins / analysis
  • Muscle Proteins / genetics*
  • MyoD Protein*
  • Myogenin
  • Nuclear Proteins / genetics*
  • Phosphoproteins / genetics*
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • Rhabdomyosarcoma / genetics*
  • Rhabdomyosarcoma / pathology
  • Rhabdomyosarcoma / ultrastructure
  • Tumor Cells, Cultured


  • Genetic Markers
  • MYOG protein, human
  • Muscle Proteins
  • MyoD Protein
  • MyoD1 myogenic differentiation protein
  • Myogenin
  • Nuclear Proteins
  • Phosphoproteins
  • RNA, Messenger