Use of gene probes based on the insertion sequence IS986 to differentiate between BCG vaccine strains

J Appl Bacteriol. 1992 Feb;72(2):126-33. doi: 10.1111/j.1365-2672.1992.tb01813.x.

Abstract

Gene probes derived from the insertion sequence IS986, which have previously been shown to differentiate isolates of Mycobacterium tuberculosis for epidemiological analysis, are also capable of distinguishing two groups of BCG vaccine strains. Most BCG strains have a single copy of IS986, at the same chromosomal site, while the Brazilian, Japanese and USSR strains have an additional copy at a different, common location. These results correlate with the results of previous antigenic analysis and may reflect a different clonal origin of the two groups of BCG strains.

MeSH terms

  • BCG Vaccine / genetics*
  • Base Sequence
  • Blotting, Southern
  • DNA Probes* / chemistry
  • DNA Transposable Elements*
  • DNA, Bacterial / chemistry*
  • Molecular Sequence Data
  • Mycobacterium bovis / genetics*
  • Nucleic Acid Hybridization
  • Polymerase Chain Reaction
  • Restriction Mapping

Substances

  • BCG Vaccine
  • DNA Probes
  • DNA Transposable Elements
  • DNA, Bacterial