Cloned platelet thrombin receptor is necessary for thrombin-induced platelet activation

J Clin Invest. 1992 Apr;89(4):1350-3. doi: 10.1172/JCI115721.


Platelet activation by thrombin is critical for hemostasis and thrombosis. Structure-function studies with a recently cloned platelet thrombin receptor suggest that a hirudin-like domain in the receptor's extracellular amino terminal extension is a thrombin-binding determinant important for receptor activation. We now report that a peptide antiserum to this domain is a potent and specific antagonist of thrombin-induced platelet activation. This study demonstrates that the cloned platelet thrombin receptor is necessary for platelet activation by thrombin, and provides a strategy for developing blocking monoclonal antibodies of potential therapeutic value.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Female
  • Hirudins
  • Humans
  • Molecular Sequence Data
  • Platelet Activation / drug effects*
  • Receptors, Cell Surface / physiology*
  • Receptors, Thrombin
  • Thrombin / pharmacology*
  • Xenopus laevis


  • Hirudins
  • Receptors, Cell Surface
  • Receptors, Thrombin
  • Thrombin