In vitro glycated low-density lipoprotein interaction with human monocyte-derived macrophages

Res Immunol. 1992 Jan;143(1):17-23. doi: 10.1016/0923-2494(92)80075-v.

Abstract

Human low-density lipoprotein (LDL) was glycated in vitro (5 days, glucose 50 mmol/l), labelled with 125I, and its binding and uptake by human monocyte-derived macrophages studied. Glycation produced lower binding and lower uptake. Competition experiments using unlabelled LDL (control, glycated, and acetyl-LDL) showed that most glycated LDL was taken up by the apolipoprotein-B100: E receptor pathway. Results suggest that less of the glycated LDL may enter the cells via scavenger receptors, and very minute amount via non-saturable receptor-independent pathways.

MeSH terms

  • Binding, Competitive
  • Biological Transport, Active
  • Glycosylation
  • Humans
  • In Vitro Techniques
  • Kinetics
  • Lipoproteins, LDL / metabolism*
  • Macrophages / metabolism*
  • Monocytes / metabolism
  • Receptors, Cell Surface / metabolism
  • Receptors, Lipoprotein*

Substances

  • Lipoproteins, LDL
  • Receptors, Cell Surface
  • Receptors, Lipoprotein
  • acetyl-LDL
  • apolipoprotein B,E receptor
  • glycosylated lipoproteins, LDL