Possible involvement of superoxide anion in the induction of cyanide-resistant respiration in Hansenula anomala

FEBS Lett. 1992 May 18;302(3):217-9. doi: 10.1016/0014-5793(92)80444-l.


A chemiluminescence study showed that Qi site inhibitors such as antimycin A induce O2- generation in respiring cyanide-sensitive mitochondria from the yeast, Hansenula anomala. The O2- generation was suppressed by radical scavengers such as flavone, butylated hydroxyanisole, and Co0. Induction of cyanide-resistant respiration in H. anomala cells by Qi site inhibitors was also inhibited by these radical scavengers. Furthermore, antimycin A-induced synthesis of the mitochondrial 36-kDa protein, which is thought to be the alternative oxidase functional in the cyanide-resistant respiratory pathway, was abolished by the addition of flavone. These observations suggest that O2- is somehow involved in the induction of cyanide-resistant respiration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anions
  • Antimycin A / pharmacology
  • Antioxidants / pharmacology
  • Cyanides / pharmacology*
  • Flavanones*
  • Flavones
  • Flavonoids / pharmacology
  • Free Radical Scavengers
  • Luminescence
  • Oxygen Consumption / drug effects*
  • Pichia / drug effects
  • Pichia / metabolism*
  • Superoxides / metabolism*


  • Anions
  • Antioxidants
  • Cyanides
  • Flavanones
  • Flavones
  • Flavonoids
  • Free Radical Scavengers
  • Superoxides
  • Antimycin A
  • flavone
  • flavanone