Inhibition of bovine papillomavirus plasmid DNA replication by adeno-associated virus

P L Hermonat

doi:10.1016/0042-6822(92)90710-7

Inhibition of bovine papillomavirus plasmid DNA replication by adeno-associated virus

Virology. 1992 Jul;189(1):329-33. doi: 10.1016/0042-6822(92)90710-7.

Author

P L Hermonat¹

Affiliation

¹ Department of Obstetrics and Gynecology, University of Arkansas for Medical Sciences, Little Rock 72205.

PMID: 1318608
DOI: 10.1016/0042-6822(92)90710-7

Abstract

The helper-dependent human parvovirus adeno-associated virus type 2 (AAV) inhibits both the oncogenic transforming abilities and the DNA replication of its helper viruses, adenovirus (Ad), and herpes simplex virus (HSV). As AAV-2 also inhibits the transforming ability of bovine papillomavirus type 1 (BPV), AAV-2 was assayed for its ability to inhibit BPV plasmid DNA replication. Here we find that the AAV-2 Rep78 gene is able to trans-inhibit BPV plasmid DNA replication and that the AAV-2 terminal repeats (TR) are also cis-required for the full inhibitory effect of AAV-2. When both the AAV-2 Rep78 open reading frame and TRs are present the inhibition of BPV plasmid DNA replication is very strong.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bovine papillomavirus 1 / growth & development*
Bovine papillomavirus 1 / pathogenicity
DNA Replication*
Dependovirus / genetics*
Gene Expression Regulation, Viral*
Helper Viruses / metabolism
Mice
Plasmids* / genetics