Cloning and sequencing of the structural region and expression of putative core gene of hepatitis C virus from a British case of chronic sporadic hepatitis

J Gen Virol. 1992 Jun;73 ( Pt 6):1521-5. doi: 10.1099/0022-1317-73-6-1521.

Abstract

We report the cloning and sequencing of the putative structural region of the hepatitis C virus (HCV) genome (2229 nucleotides) from an isolate derived from a British case of chronic sporadic non-A, non-B hepatitis. The overall sequence shows a higher similarity with one type of HCV, HCV1 (92%), than with HCV2 (80%), is very highly conserved at the 5' end (99%) preceding the long open reading frame, is well conserved also in the putative core region (90 to 97%), but shows marked variation in the putative envelope region, particularly in the envelope 2/non-structural 1 region (70%). The putative core gene was cloned in pJ3 omega under the early simian virus 40 promoter and expressed in human hepatoma cells. A predominantly cytoplasmic 22K polypeptide was expressed which was antigenically reactive with serum from chronically infected HCV patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chronic Disease
  • Cloning, Molecular
  • DNA, Viral
  • Fluorescent Antibody Technique
  • Genes, Viral*
  • Hepacivirus / genetics*
  • Hepatitis C / microbiology*
  • Humans
  • Immunoblotting
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Tumor Cells, Cultured
  • Viral Core Proteins / genetics*
  • Viral Structural Proteins / genetics*

Substances

  • DNA, Viral
  • Viral Core Proteins
  • Viral Structural Proteins