Radioprotection by vitamin E: injectable vitamin E administered alone or with WR-3689 enhances survival of irradiated mice

Int J Radiat Oncol Biol Phys. 1992;23(4):841-5. doi: 10.1016/0360-3016(92)90657-4.


Radioprotection by injectable vitamin E (alpha-tocopherol) was investigated in mice exposed to 60Co radiation (0.2 Gy/min). Vitamin E injected subcutaneously either 1 hr before or within 15 min after irradiation significantly increased 30-day postirradiation survival in CD2F1 male mice. A dose reduction factor (DRF) of 1.11 (95% confidence interval [1.08, 1.14]) was observed for vitamin E at a dose of 100 IU/kg body weight administered within 15 min after irradiation. Combination studies with the phosphorothioate WR-3689 (S-2([3-methylaminopropyl]amino)ethylphosphorothioic acid) were undertaken to determine whether radioprotection by WR-3689 could be enhanced by vitamin E. Mice were given WR-3689 (150-225 mg/kg, intraperitoneally) 30 min before irradiation and were given vitamin E (100 IU/kg) either 1 hr before or within 15 min after irradiation. Survival was significantly increased in mice given vitamin E and WR-3689 before irradiation as compared to mice given WR-3689 alone: the DRF for WR-3689 (150 mg) was 1.35 [1.32, 1.38]; for WR-3689 combined with vitamin E (100 IU), the DRF was 1.49 [1.45, 1.53].

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amifostine / administration & dosage
  • Amifostine / analogs & derivatives*
  • Amifostine / therapeutic use
  • Animals
  • Injections, Subcutaneous
  • Male
  • Mice
  • Radiation Injuries, Experimental / mortality*
  • Radiation-Protective Agents / administration & dosage
  • Radiation-Protective Agents / therapeutic use*
  • Survival Rate
  • Vitamin E / administration & dosage
  • Vitamin E / therapeutic use*


  • Radiation-Protective Agents
  • Vitamin E
  • S-2-(3-methylaminopropylamino)ethylphosphorothioic acid
  • Amifostine