Human T-cell leukemia virus-1-positive cell line established from a patient with small cell lung cancer

Jpn J Cancer Res. 1992 May;83(5):450-7. doi: 10.1111/j.1349-7006.1992.tb01949.x.


A stable cell line, KHM-3S, was established from a patient with small cell lung cancer (SCLC), who had a high serum level of soluble interleukin 2 receptors (sIL2-R) and was seropositive for human T cell leukemia virus (HTLV)-1. KHM-3S cells were positive for IL2-R (Tac) and NKH-1, but negative for other lymphocytic markers such as OKT 11, OKT 4, OKT 8, T cell receptor (WT 31), B 1, and B 4. Moreover, the KHM-3S cells were negative for leukocyte common antigen and strongly positive for neuron-specific enolase (NSE). Secretion of sIL2-R and NSE by the KHM-3S line was detected by an enzyme-linked immunosorbent assay. Rearrangement of the T cell receptor gene and monoclonal HTLV-1 integration were found by Southern blot analysis of KHM-3S DNA. However, Northern blot analysis showed no T cell receptor mRNA. KHM-3S may be useful for studies on the role of HTLV-1 in carcinogenesis and IL2-R expression in SCLC.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Surface / analysis
  • Blotting, Northern
  • Blotting, Southern
  • Carcinoma, Small Cell / microbiology*
  • Carcinoma, Small Cell / pathology
  • Carcinoma, Small Cell / physiopathology
  • HTLV-I Infections / blood
  • HTLV-I Infections / pathology*
  • HTLV-I Infections / physiopathology
  • Human T-lymphotropic virus 1 / isolation & purification*
  • Humans
  • Karyotyping
  • Lung Neoplasms / microbiology*
  • Lung Neoplasms / pathology
  • Lung Neoplasms / physiopathology
  • Male
  • Middle Aged
  • Receptors, Interleukin-2 / biosynthesis
  • Tumor Cells, Cultured


  • Antigens, Surface
  • Receptors, Interleukin-2