Insertional inactivation of a gene which controls expression of vancomycin resistance on plasmid pHKK100

FEMS Microbiol Lett. 1992 Apr 1;71(1):11-4. doi: 10.1016/0378-1097(92)90533-t.

Abstract

Expression of inducible high level vancomycin resistance (Vmr) in enterococci appears to require other plasmid-encoded genes in addition to the previously described structural genes vanA and vanH. Tn917 mutagenesis was used to identify such a region in the Vmr plasmid pHKK100. Insertional inactivation of a 693-bp open reading frame upstream from vanH resulted in complete loss of Vmr. This putative 26,642-Da protein has been designated VanR.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA Transposable Elements
  • Drug Resistance, Microbial / genetics*
  • Enterococcus / drug effects
  • Enterococcus / genetics*
  • Gene Expression
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Phenotype
  • R Factors / genetics*
  • Vancomycin / pharmacology*

Substances

  • DNA Transposable Elements
  • Vancomycin

Associated data

  • GENBANK/M84146