Comparison of a calcitonin gene-related peptide receptor in a human neuroblastoma cell line (SK-N-MC) and a calcitonin receptor in a human breast carcinoma cell line (T47D)

Ann N Y Acad Sci. 1992 Jun 30;657:106-16. doi: 10.1111/j.1749-6632.1992.tb22760.x.

Abstract

A specific CGRP-binding protein of M(r) 60,000 has been identified in the human neuroblastoma cell line SK-N-MC. After N-deglycosylation a M(r) of 48,000 was found. The M(r) were indistinguishable from those determined in the human cerebellum. Receptor binding of CGRP is coupled to cyclic AMP formation. The latter is antagonized by hCGRP-I8-37. CT and DAPamide interact only minimally with the CGRP receptor, whereas CGRP and DAPamide are full agonists in T47D cells. The CT receptor on human breast cancer cell line T47D is clearly different from the human CGRP receptor.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Breast Neoplasms
  • Calcitonin Gene-Related Peptide / metabolism*
  • Cell Line
  • Cyclic AMP / metabolism
  • Female
  • Humans
  • Kinetics
  • Molecular Sequence Data
  • Molecular Weight
  • Neuroblastoma
  • Receptors, Calcitonin
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / isolation & purification
  • Receptors, Cell Surface / metabolism*
  • Sequence Homology, Nucleic Acid

Substances

  • Receptors, Calcitonin
  • Receptors, Cell Surface
  • Cyclic AMP
  • Calcitonin Gene-Related Peptide