Chicken "Erythroid" Cells Transformed by the Gag-Myb-Ets-encoding E26 Leukemia Virus Are Multipotent

Cell. 1992 Jul 24;70(2):201-13. doi: 10.1016/0092-8674(92)90096-u.

Abstract

The E26 avian leukemia virus encodes a transcriptional activator-type oncoprotein consisting of Gag, Myb, and Ets domains, and transforms early erythroid cells as well as myeloblasts. Surprisingly, we have found that "early erythroid" transformants obtained in culture are multipotent, since they can be induced to differentiate into myeloblasts and eosinophils after superinfection with retroviruses containing kinase-type or ras oncogenes. In addition, TPA is an efficient inducer that generates predominantly eosinophils at low concentrations and myeloblasts at high concentrations. The determination process involves the complete extinction of erythroid/thrombocytic markers and the subsequent activation of myelomonocytic/eosinophilic properties, including the acquisition of specific growth factor requirements. "Erythroleukemic" cells from virus-infected animals were likewise found to be multipotent, making this a unique system to study the genesis of stem cell leukemias and the molecular basis of lineage commitment during hematopoiesis.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Avian Leukosis Virus
  • Base Sequence
  • Cell Differentiation / drug effects
  • Chick Embryo
  • Clone Cells
  • Eosinophils / microbiology*
  • Eosinophils / ultrastructure
  • Genes, ras / physiology*
  • Hematopoiesis* / drug effects
  • Molecular Sequence Data
  • Phorbol Esters / pharmacology

Substances

  • Antibodies, Monoclonal
  • Phorbol Esters