Mouse peritoneal macrophage prostaglandin E1 synthesis is altered by dietary gamma-linolenic acid

J Nutr. 1992 Aug;122(8):1600-6. doi: 10.1093/jn/122.8.1600.

Abstract

The ability of dietary gamma-linolenic acid [18:3(n-6)] to modulate prostaglandin biosynthesis in mouse resident peritoneal macrophages was determined. Mice were fed diets containing corn oil, borage oil or evening primrose oil or a mixture of borage and fish oils. After 2 wk, resident peritoneal macrophages were isolated and stimulated with unopsonized zymosan to induce prostaglandin synthesis. Borage oil, primrose oil and fish-borage oil mixture dietary groups (containing 25.6, 11.9 and 19.5 g gamma-linolenic acid/100 g fatty acids, respectively) had significantly (P less than 0.05) enhanced prostaglandin E1 synthesis (39.7, 29.4 and 73.0 nmol prostaglandin E1/mg protein, respectively) compared with corn oil-fed (containing less than 0.1 g gamma-linolenic acid/100 g fatty acids) animals, which synthesized less than 0.1 nmol prostaglandin E1/mg protein. Borage oil- and fish-borage oil mixture-fed mice had the highest biosynthetic ratio of prostaglandin E1/prostaglandin E2 (E1/E2 approximately 0.2). Macrophages from borage oil-fed mice synthesized the lowest amount of prostacyclin (198.7 nmol 6-keto-prostaglandin F1 alpha/mg protein) compared with corn oil-, primrose oil- and fish-borage oil mixture-fed mice (379.7, 764.8 and 384.2 nmol 6-keto-prostaglandin F1 alpha/mg protein, respectively). In addition, borage oil-, primrose oil- and fish-borage oil mixture-fed mice had significantly (P less than 0.05) higher levels of dihomo-gamma-linolenic acid [20:3(n-6)] in membrane phospholipids (5.5, 3.5 and 5.7 mol/100 mol, respectively) relative to corn oil-fed mice (2.0 mol/100 mol).(ABSTRACT TRUNCATED AT 250 WORDS)

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alprostadil / biosynthesis
  • Animals
  • Ascitic Fluid / cytology
  • Dietary Fats, Unsaturated / administration & dosage
  • Dietary Fats, Unsaturated / pharmacology*
  • Dinoprostone / biosynthesis
  • Epoprostenol / biosynthesis
  • Fatty Acids, Essential / pharmacology
  • Female
  • Fish Oils / pharmacology
  • Linoleic Acids
  • Linolenic Acids / administration & dosage
  • Linolenic Acids / pharmacology*
  • Macrophages / drug effects
  • Macrophages / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Phospholipids / metabolism
  • Plant Oils / pharmacology
  • Zymosan / pharmacology
  • gamma-Linolenic Acid

Substances

  • Dietary Fats, Unsaturated
  • Fatty Acids, Essential
  • Fish Oils
  • Linoleic Acids
  • Linolenic Acids
  • Phospholipids
  • Plant Oils
  • evening primrose oil
  • gamma-Linolenic Acid
  • Zymosan
  • Epoprostenol
  • Alprostadil
  • Dinoprostone