Affinity chromatographic purification of bovine lung endothelin receptor using biotinylated endothelin and avidin-agarose

J Chromatogr. 1992 Apr 24;597(1-2):331-4. doi: 10.1016/0021-9673(92)80128-h.

Abstract

Endothelin receptor was purified from bovine lung by affinity chromatography using biotinylated endothelin and avidin-agarose. Endothelin was biotinylated with sulphosuccinimidyl 2-(biotinamido)ethyl-1,3-dithiopropionate, a reactive form of biotin with a cleavable spacer arm containing a disulphide bond designed for a simple elution by thiols. Starting from 3.5 kg of bovine lung, about 200 micrograms of pure receptor were obtained.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Avidin
  • Biotin
  • Cattle
  • Chromatography, Affinity
  • Electrophoresis, Polyacrylamide Gel
  • Endothelins
  • Lung / chemistry*
  • Molecular Sequence Data
  • Receptors, Cell Surface / isolation & purification*
  • Receptors, Endothelin
  • Sepharose

Substances

  • Endothelins
  • Receptors, Cell Surface
  • Receptors, Endothelin
  • Avidin
  • Biotin
  • Sepharose