Abstract
Rat surfactant protein D (SP-D) was shown to enhance the production of oxygen radicals by rat alveolar macrophages. This enhancement, which was determined by a lucigenin-dependent chemiluminescence assay, was maximal after 18 min at an SP-D concentration of 0.2 micrograms/ml. Surfactant lipids did not influence the stimulation of alveolar macrophages by SP-D, whereas the oxygen-radical production of these cells induced by surfactant protein A was inhibited by the lipids in a concentration-dependent manner.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bronchoalveolar Lavage Fluid
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Glycoproteins / isolation & purification
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Glycoproteins / pharmacology*
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Humans
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Kinetics
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Luminescent Measurements
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Macrophages / drug effects
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Macrophages / metabolism
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Macrophages, Alveolar / drug effects
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Macrophages, Alveolar / metabolism*
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Phospholipids / pharmacology
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Proteolipids / isolation & purification
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Proteolipids / pharmacology
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Pulmonary Surfactant-Associated Protein D
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Pulmonary Surfactant-Associated Proteins
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Pulmonary Surfactants / isolation & purification
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Pulmonary Surfactants / pharmacology*
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Rats
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Superoxides / metabolism*
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Tetradecanoylphorbol Acetate / pharmacology
Substances
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Glycoproteins
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Phospholipids
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Proteolipids
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Pulmonary Surfactant-Associated Protein D
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Pulmonary Surfactant-Associated Proteins
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Pulmonary Surfactants
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Superoxides
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Tetradecanoylphorbol Acetate