Induction of differentiation of the human promyelocytic cell line HL-60 by activin/EDF

Biochem Biophys Res Commun. 1992 Aug 31;187(1):79-85. doi: 10.1016/s0006-291x(05)81461-5.


A human promyelocytic cell line, HL-60, treated with activin/EDF was found to differentiate into monocyte/macrophage-like cells. This was shown not only by morphology but by the loss of myeloperoxidase granules and the appearance of nonspecific esterase. Dose-dependent inhibition of the differentiation by follistatin, an activin-binding protein, confirmed that it was indeed caused by activin. Thus, activin/EDF exerts its effect on hematopoietic cells not only on erythroid differentiation but also on at least a part of myeloid cell differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Activins
  • Binding Sites
  • Cell Differentiation / drug effects
  • Cytoplasmic Granules / ultrastructure
  • Epidermal Growth Factor / pharmacology*
  • Follistatin
  • Glycoproteins / pharmacology
  • Histocytochemistry
  • Humans
  • Inhibins / metabolism
  • Inhibins / pharmacology*
  • Leukemia, Promyelocytic, Acute / metabolism
  • Leukemia, Promyelocytic, Acute / pathology*
  • Peroxidase / metabolism
  • Tumor Cells, Cultured


  • Follistatin
  • Glycoproteins
  • Activins
  • Inhibins
  • Epidermal Growth Factor
  • Peroxidase