Molecular cloning of a new bombesin receptor subtype expressed in uterus during pregnancy

Eur J Biochem. 1992 Sep 1;208(2):405-10. doi: 10.1111/j.1432-1033.1992.tb17201.x.


The homology screening approach has been used to clone a new member of the guanine-nucleotide-binding-protein-coupled receptor superfamily from guinea pig uterus. The cloned cDNA encodes a 399-amino-acid protein and shows the highest amino acid similarity to members of the bombesin receptor family; 52% and 47% similarity to the gastrin-releasing-peptide (GRP) receptor and the neuromedin-B receptor, respectively. Binding experiments with the stably transfected LLC-PK1 cell line expressing the new receptor protein confirmed the bombesin-like nature of the cloned receptor. The relative order of ligand affinity, GRP = neuromedin C much greater than neuromedin B, suggests that the cloned cDNA represents the GRP subtype rather than the neuromedin-B subtype of bombesin receptors. Northern-blot analysis of mRNA species from several guinea-pig tissues showed that the mRNA for the new bombesin receptor subtype is expressed mainly in uteri of pregnant animals.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cloning, Molecular*
  • DNA / chemistry
  • DNA / isolation & purification
  • Female
  • Gene Expression
  • Guinea Pigs
  • Kidney
  • Molecular Sequence Data
  • Pregnancy
  • Pregnancy, Animal / metabolism*
  • RNA, Messenger / metabolism
  • Receptors, Bombesin
  • Receptors, Neurotransmitter / chemistry
  • Receptors, Neurotransmitter / genetics*
  • Sequence Homology, Nucleic Acid
  • Transfection
  • Uterus / metabolism*


  • RNA, Messenger
  • Receptors, Bombesin
  • Receptors, Neurotransmitter
  • DNA

Associated data

  • GENBANK/X67126