Determination of a cyclic heptapeptide, a novel fibrinogen receptor antagonist, in human plasma by high-performance liquid chromatography with automated pre-column derivatization, column switching and fluorescence detection

J Chromatogr. 1992 Jul 1;578(1):31-7. doi: 10.1016/0378-4347(92)80221-b.


A sensitive high-performance liquid chromatographic (HPLC) assay for the determination of the cyclic heptapeptide Ac-Cs-Asn-Dtc-Amf-Gly-Asp-Cys-OH (Dtc = beta,beta-dimethylthioproline, Amf = p-aminomethylphenylalanine) in human plasma has been developed. The key steps in the assay include: solid-phase extraction of the drug from plasma, chemical derivatization of the primary amino group with naphthalene-2,3-dicarboxyaldehyde in the presence of N-acetyl-D-penicillamine as a nucleophile to form a fluorescent benzo[f]isoindole derivative, and HPLC with column switching to provide the necessary chromatographic separation of the derivative from endogenous plasma components. The assay has been validated in the concentration range 1-10 ng/ml of plasma.

MeSH terms

  • Amino Acid Sequence
  • Chromatography, High Pressure Liquid / instrumentation
  • Chromatography, High Pressure Liquid / methods*
  • Humans
  • Molecular Sequence Data
  • Naphthalenes
  • Peptides, Cyclic / blood*
  • Peptides, Cyclic / isolation & purification
  • Peptides, Cyclic / pharmacology
  • Platelet Aggregation Inhibitors / blood
  • Receptors, Cell Surface / antagonists & inhibitors*
  • Receptors, Peptide*


  • Naphthalenes
  • Peptides, Cyclic
  • Platelet Aggregation Inhibitors
  • Receptors, Cell Surface
  • Receptors, Peptide
  • fibrin-fibrinogen degradation products receptor
  • cyclo(N-acetylcysteinyl-asparaginyl-dimethylthioprolyl-(4-aminomethyl)phenylalanyl-glycyl-aspartyl-cysteine)
  • 2,3-naphthalenedicarboxaldehyde