Glycoprotein H of human cytomegalovirus (HCMV) forms a stable complex with the HCMV UL115 gene product

J Gen Virol. 1992 Oct;73 ( Pt 10):2693-8. doi: 10.1099/0022-1317-73-10-2693.

Abstract

The human cytomegalovirus (HCMV) UL75 gene product is the homologue of herpes simplex virus type 1 (HSV-1) glycoprotein H (gH), a virion glycoprotein that is essential for infectivity and which is conserved among members of the alpha-, beta- and gamma-herpesviruses. It has previously been shown that HSV-1 gH forms a stable complex with HSV-1 gL, the product of the UL1 gene, and the formation of this complex facilitates the cell surface expression of gH. None of the open reading frames within the HCMV genome encode a product with discernible sequence homology with HSV-1 gL, but an examination of the arrangement of conserved genes in HCMV suggested that the UL115 gene is a 'positional homologue' of HSV-1 UL1 which, like UL1, encodes a small secreted glycoprotein. Co-expression of HCMV gH (the UL75 gene product) and the UL115 gene product revealed that these proteins form a disulphide-linked complex and that the formation of this complex results in cell surface expression of gH. This complex is analogous to the gH:gL complex of HSV-1 and the HCMV UL115 gene product is therefore the functional homologue of HSV-1 gL.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Cytomegalovirus / genetics
  • Cytomegalovirus / metabolism*
  • Fluorescent Antibody Technique
  • Humans
  • Sequence Analysis, DNA
  • Viral Envelope Proteins / genetics
  • Viral Envelope Proteins / isolation & purification
  • Viral Envelope Proteins / metabolism*
  • Viral Proteins / metabolism*

Substances

  • UL115 protein, Human herpesvirus 5
  • Viral Envelope Proteins
  • Viral Proteins
  • glycoprotein H, herpes simplex virus type 1
  • glycoprotein L, Human herpesvirus 1