Molecular cloning and characterization of two lincomycin-resistance genes, lmrA and lmrB, from Streptomyces lincolnensis 78-11

Mol Microbiol. 1992 Aug;6(15):2147-57. doi: 10.1111/j.1365-2958.1992.tb01388.x.


Two different lincomycin-resistance determinants (lmrA and lmrB) from Streptomyces lincolnensis 78-11 were cloned in Streptomyces lividans 66 TK23. The gene lmrA was localized on a 2.16 kb fragment, the determined nucleotide sequence of which encoded a single open reading frame 1446 bp long. Analysis of the deduced amino acid sequence suggested the presence of 12 membrane-spanning domains and showed significant similarities to the methylenomycin-resistance protein (Mmr) from Streptomyces coelicolor, the QacA protein from Staphylococcus aureus, and several tetracycline-resistance proteins from both Gram-positive and Gram-negative bacteria, as well as to some sugar-transport proteins from Escherichia coli. The lmrB gene was actively expressed from a 2.7 kb fragment. An open reading frame of 837 bp could be localized which encoded a protein that was significantly similar to 23S rRNA adenine(2058)-N-methyltransferases conferring macrolide-lincosamide-streptogramin resistance. LmrB also had putative rRNA methyltransferase activity since lincomycin resistance of ribosomes was induced in lmrB-containing strains. Surprisingly, both enzymes, LmrA and LmrB, had a substrate specificity restricted to lincomycin and did not cause resistance to other lincosamides such as celesticetin and clindamycin, or to macrolides.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Carrier Proteins / genetics
  • Catalysis
  • Cloning, Molecular
  • DNA, Bacterial / isolation & purification
  • Drug Resistance, Microbial / genetics
  • Gene Expression Regulation, Bacterial
  • Genes, Bacterial*
  • Lincomycin / pharmacology*
  • Methylation
  • Methyltransferases / genetics
  • Molecular Sequence Data
  • Phenotype
  • Protons
  • RNA, Ribosomal, 23S / genetics
  • Sequence Homology
  • Streptomyces / drug effects
  • Streptomyces / genetics*


  • Carrier Proteins
  • DNA, Bacterial
  • Protons
  • RNA, Ribosomal, 23S
  • Lincomycin
  • Methyltransferases

Associated data

  • GENBANK/X59926
  • GENBANK/X62867