Glycosaminoglycans and proteoglycans of human chondrosarcoma

Biochim Biophys Acta. 1976 Jun 23;437(1):71-86. doi: 10.1016/0304-4165(76)90348-2.

Abstract

The glycosaminoglycans and proteoglycans of a human chondrosarcoma have been studied. Glycosaminoglycans were fractionated and identified by cetylpirdium chloride (CPC) cellulose chromatography, ECTEOLA cellulose ion-exchange chromatography and electrophoresis on cellulose acetate. Proteoglycans were extracted by low ionic strength solutions and by 4 M guanidinium chloride and fractioned by equilibrium density-gradient centrifugation and gel chromatography on Sepharose 2B. The tumour matrix contained both the 4- and 6-sulphate isomers of chondroitin sulphate and a high concentration (12% of hexosamine) of hyaluronic acid. Proteoglycans were poor in carbohydrate moieties and proportion were capable of aggregation. Amino acid analysis of the fractionated proteoglycans suggested the presence of a single protein core. A substance with the characteristic amino acid composition of glycoprotein link was recovered from the top of the dissociative density gradient.

Publication types

  • Case Reports

MeSH terms

  • Amino Acids / analysis
  • Chondrosarcoma / metabolism*
  • Galactosamine / analysis
  • Glucosamine / analysis
  • Glycosaminoglycans* / isolation & purification
  • Glycosaminoglycans* / metabolism
  • Humans
  • Male
  • Middle Aged
  • Papain
  • Proteoglycans* / isolation & purification
  • Proteoglycans* / metabolism
  • Spectrophotometry, Infrared
  • Uronic Acids / analysis

Substances

  • Amino Acids
  • Glycosaminoglycans
  • Proteoglycans
  • Uronic Acids
  • Galactosamine
  • Papain
  • Glucosamine