The lower matrix protein pp65 is the principal viral antigen present in peripheral blood leukocytes during an active cytomegalovirus infection

J Gen Virol. 1992 Nov;73 ( Pt 11):2923-32. doi: 10.1099/0022-1317-73-11-2923.

Abstract

During an active infection with human cytomegalovirus (HCMV), viral antigen is consistently present in peripheral blood leukocytes. Two monoclonal antibodies (MAbs), CMV-C10 and CMV-C11, are commonly used in the HCMV antigenaemia assay to detect these cells in the peripheral blood of patients suspected of having an active HCMV infection. We demonstrate that the viral antigen detected by these MAbs is the viral structural protein pp65 and not an immediate early antigen as previously reported. Furthermore, significantly fewer leukocytes were found to be positive with MAbs specific for immediate early antigens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal
  • Antibodies, Viral / immunology
  • Antibody Specificity
  • Antigens, Viral / immunology*
  • Antigens, Viral / isolation & purification
  • Cell Nucleus / immunology
  • Cell Nucleus / metabolism
  • Cytomegalovirus Infections / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Fluorescent Antibody Technique
  • Humans
  • Immediate-Early Proteins*
  • Leukocytes / immunology*
  • Leukocytes, Mononuclear / immunology
  • Phosphoproteins / biosynthesis
  • Phosphoproteins / genetics
  • Phosphoproteins / immunology*
  • Phosphoproteins / isolation & purification
  • Precipitin Tests
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / immunology
  • Viral Matrix Proteins / biosynthesis
  • Viral Matrix Proteins / genetics
  • Viral Matrix Proteins / immunology*
  • Viral Matrix Proteins / isolation & purification
  • Viral Structural Proteins / biosynthesis
  • Viral Structural Proteins / genetics
  • Viral Structural Proteins / immunology*
  • Viral Structural Proteins / isolation & purification

Substances

  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Antigens, Viral
  • Immediate-Early Proteins
  • Phosphoproteins
  • Recombinant Proteins
  • Viral Matrix Proteins
  • Viral Structural Proteins
  • cytomegalovirus matrix protein 65kDa
  • immediate-early proteins, cytomegalovirus