Detection of JC virus in the brains of aged patients without progressive multifocal leukoencephalopathy by the polymerase chain reaction and Southern hybridization analysis

Neurosci Lett. 1992 Jul 20;141(2):151-5. doi: 10.1016/0304-3940(92)90883-9.

Abstract

Ninety-one brain tissue sections taken at autopsy from 33 elderly patients (63-100 years old) without progressive multifocal leukoencephalopathy were examined for the presence of JC virus DNA by the polymerase chain reaction and Southern hybridization analysis after DNA extraction. JC virus DNA was detected in 15 sections from 10 patients. These results suggest that JC virus is frequently present in the brains of aged patients.

MeSH terms

  • Aged
  • Aged, 80 and over
  • Aging
  • Base Sequence
  • Brain / embryology
  • Brain / metabolism*
  • DNA, Viral / analysis
  • Fetus / metabolism
  • Humans
  • JC Virus / isolation & purification*
  • Leukoencephalopathy, Progressive Multifocal / microbiology
  • Middle Aged
  • Molecular Probes / genetics
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Polymerase Chain Reaction

Substances

  • DNA, Viral
  • Molecular Probes