Mapping of GABAA receptor alpha 5 and alpha 6 subunit-like immunoreactivity in rat brain

Neurosci Lett. 1992 Sep 14;144(1-2):53-6. doi: 10.1016/0304-3940(92)90714-i.


The distribution of the alpha 5 and alpha 6 subunits of the GABAA receptor has been mapped in rat brain using affinity-purified antibodies generated against peptide sequences unique to the respective polypeptides. alpha 5 Subunit-like immunoreactivity was of low density but was distributed across several cell groups including cortical interneurones, hippocampal CA3 pyramidal neurones, the anterior thalamic reticular nucleus and cerebellar Purkinje neurones. alpha 6 Subunit-like immunoreactivity was observed in high density in cerebellar granule cells. These patterns are compatible with in situ hybridisation studies and provide a further anatomical substrate for GABAA receptor heterogeneity in the CNS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Brain / metabolism*
  • Brain Mapping*
  • Immunohistochemistry
  • Molecular Sequence Data
  • RNA, Messenger / biosynthesis
  • Rats
  • Receptors, GABA-A / metabolism*


  • RNA, Messenger
  • Receptors, GABA-A