The Virulence-Determining Genomic BamHI Fragment 4 of Pseudorabies Virus Contains Genes Corresponding to the UL15 (Partial), UL18, UL19, UL20, and UL21 Genes of Herpes Simplex Virus and a Putative Origin of Replication

Virology. 1992 Dec;191(2):900-8. doi: 10.1016/0042-6822(92)90265-q.

Abstract

The genomic BamHI-fragment 4 of pseudorabies virus (PrV) has previously been shown to encode functions necessary for expression of PrV neurovirulence (B. Lomniczi, S. Watanabe, T. Ben-Porat, and A. S. Kaplan, 1984, J. Virol. 52, 198-205). To identify proteins that might be involved in the neurotropism of PrV we sequenced the complete 9382-bp fragment BamHI-4, the longest contiguous sequence determined in the UL region of PrV so far, and analyzed its coding capacity. In an arrangement similar to that found in herpes simplex virus type 1 we identified complete open reading frames encoding proteins with strong homology to the UL18 (50% homology), UL19 (60% homology), UL20 (33% homology), and UL21 (36% homology) polypeptides and the 3'-part of a gene homologous to UL15 (67% homology) of HSV-1. In addition, a consensus sequence for an alphaherpesviral origin of replication was found at the left terminus of the fragment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Chromosome Mapping
  • Consensus Sequence
  • DNA Replication
  • DNA, Recombinant / genetics
  • DNA, Viral / drug effects
  • DNA, Viral / genetics
  • Deoxyribonuclease BamHI / metabolism
  • Genes, Viral / genetics*
  • Herpesvirus 1, Suid / genetics*
  • Herpesvirus 1, Suid / pathogenicity*
  • Molecular Sequence Data
  • Open Reading Frames
  • Sequence Homology, Nucleic Acid
  • Simplexvirus / genetics
  • Viral Proteins / genetics
  • Virulence / genetics*

Substances

  • DNA, Recombinant
  • DNA, Viral
  • Viral Proteins
  • Deoxyribonuclease BamHI

Associated data

  • GENBANK/L00676