Detection of p53 gene mutations in human ovarian and endometrial cancers by polymerase chain reaction-single strand conformation polymorphism analysis

Jpn J Cancer Res. 1992 Oct;83(10):1030-6. doi: 10.1111/j.1349-7006.1992.tb02717.x.

Abstract

The presence of mutations in the p53 gene was examined in ovarian cancers by a polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis. The primers were designed to amplify exons 5 through 9 that contain phylogenetically conserved domains of the p53 gene. Mutations were detected in 5 out of 10 cases, one of which contained a deletion in the second allele. A single base substitution was detected in 4 cases at codons 162, 175, 205 and 273 and a single base insertion in one case within codon 315. A high frequency of p53 mutations in ovarian cancers and lack of mutation in 6 benign ovarian tumors and 2 normal ovaries suggested that the mutation of the p53 gene was associated with the genesis and/or progression of ovarian cancer. In 1 of 7 endometrial cancers, two mutations at codons 239 and 254 were detected.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Base Sequence
  • Chromosome Deletion
  • DNA / analysis
  • DNA / genetics
  • DNA, Neoplasm / analysis*
  • DNA, Neoplasm / genetics
  • DNA, Single-Stranded / analysis*
  • DNA, Single-Stranded / genetics
  • DNA, Viral / analysis
  • Endometrial Neoplasms / genetics*
  • Endometrial Neoplasms / microbiology
  • Endometrial Neoplasms / secondary
  • Exons / genetics
  • Female
  • Genes, p53 / genetics*
  • Genome, Viral
  • Humans
  • Molecular Sequence Data
  • Mutation
  • Nucleic Acid Conformation
  • Ovarian Neoplasms / genetics*
  • Ovarian Neoplasms / microbiology
  • Ovarian Neoplasms / secondary
  • Papillomaviridae / genetics
  • Placenta / chemistry
  • Placenta / physiology
  • Polymerase Chain Reaction / methods*
  • Polymorphism, Genetic / genetics*
  • Pregnancy

Substances

  • DNA, Neoplasm
  • DNA, Single-Stranded
  • DNA, Viral
  • DNA