A noninvasive assessment of myocardial oxygen tension: 19F NMR spectroscopy of sequestered perfluorocarbon emulsion

Magn Reson Med. 1992 Oct;27(2):310-7. doi: 10.1002/mrm.1910270210.


Fluorine NMR spectroscopy of sequestered perfluorocarbon emulsion has been used to measure myocardial oxygen tension. This novel application provides a rapid noninvasive assessment of changes in oxygen tension in response to ischemia and reperfusion. Rats were predosed with Oxypherol-ET (emulsion of perfluorotributylamine). Following vascular clearance of the emulsion the heart was excised and perfused using the Langendorff retrograde technique. 19F spin-lattice relaxation time measurements provided an accurate estimate of myocardial pO2. Using a two-point determination with a time resolution of 1 s, the loss of oxygen was found to be complete within 40 s of the onset of global ischemia. The fall in oxygen tension correlated closely with an observed loss of ventricular pressure. Magnetic resonance imaging showed that perfluorocarbon was distributed throughout the heart; thus, this reporter molecule provides a global measurement of oxygen tension.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Blood Substitutes*
  • Electron Spin Resonance Spectroscopy
  • Emulsions
  • Fluorine
  • Fluorocarbons*
  • Magnetic Resonance Imaging
  • Magnetic Resonance Spectroscopy*
  • Myocardial Ischemia / metabolism
  • Myocardial Reperfusion
  • Myocardium / metabolism*
  • Oxygen Consumption*
  • Rats
  • Rats, Sprague-Dawley


  • Blood Substitutes
  • Emulsions
  • Fluorocarbons
  • Fluorine
  • perfluorotributylamine