Enhancement of hippocampal excitatory synaptic transmission by platelet-activating factor

Neuron. 1992 Dec;9(6):1211-6. doi: 10.1016/0896-6273(92)90078-r.

Abstract

The biologically active lipid platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphorylcholine; PAF) is a mediator of inflammatory and immune responses, and it accumulates in the brain during convulsions or ischemia. We have examined whether PAF may play a second messenger role in the central nervous system by studying effects on synaptic transmission in cultured hippocampal neurons. Carbamyl-PAF, a nonhydrolyzable PAF analog with a similar pharmacologic profile, augmented glutamate-mediated, evoked excitatory synaptic transmission and increased the frequency of spontaneous miniature excitatory synaptic events without increasing their amplitude or altering their time course. This compound had no significant effect on gamma-aminobutyric acid-mediated inhibitory synaptic responses. Lyso-PAF, the biologically inactive metabolic intermediate, had no effect on synaptic transmission. Moreover, the enhancement of excitatory synaptic transmission by carbamyl-PAF was blocked by a PAF receptor antagonist. These results indicate a specific presynaptic effect of PAF in enhancing excitatory synaptic transmission in cultured rat hippocampal neurons.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Animals, Newborn
  • Bicuculline / pharmacology
  • Evoked Potentials / drug effects
  • Hippocampus / drug effects
  • Hippocampus / physiology*
  • Kinetics
  • Organ Culture Techniques
  • Platelet Activating Factor / pharmacology*
  • Rats
  • Synapses / drug effects
  • Synapses / physiology*
  • Synaptic Transmission / drug effects*
  • Time Factors
  • gamma-Aminobutyric Acid / pharmacology

Substances

  • Platelet Activating Factor
  • gamma-Aminobutyric Acid
  • Bicuculline