Modified Binary Plant Transformation Vectors With the Wild-Type Gene Encoding NPTII

Gene. 1992 Dec 15;122(2):383-4. doi: 10.1016/0378-1119(92)90232-e.

Abstract

The defective gene encoding neomycin phosphotransferase (NPTII) present in the binary plasmid vector, pBin19, was replaced with the wild-type (wt) gene. Plasmid vectors analogous to pBin19, pBI121 and pBI101 were constructed carrying the gene encoding the wt NPTII enzyme activity.

MeSH terms

  • Genetic Vectors*
  • Kanamycin Kinase
  • Phosphotransferases / genetics*
  • Plants / genetics*
  • Plasmids*
  • Transformation, Genetic*

Substances

  • Phosphotransferases
  • Kanamycin Kinase