C-fos expression in the rat brain after intraperitoneal injection of lithium chloride

Neuroreport. 1992 Dec;3(12):1049-52. doi: 10.1097/00001756-199212000-00004.


The distribution of evoked expression of the proto-oncogene c-fos was immunohistochemically examined in the rat brain after intraperitoneal injection of isotonic LiCl, which is commonly used to induce internal malaise in the conditioned taste aversion paradigm. C-fos-like immunoreactive neurones (c-fos neurones) were most densely observed in the central amygdaloid nucleus, external lateral subnucleus of the parabrachial nucleus (PBN), posteromedial and commissural parts of the nucleus of the tractus solitarius (NTS) and area postrema (AP). Experiments including vagotomy, intravenous injection of LiCl and lesions of the area postrema suggest that NTS neurones are activated via both sides of the vagus nerves, while AP neurones, humorally as well as neurally via the vagal nerve with a right side predominance. The activated NTS and AP neurones project mainly to the external lateral subnucleus of the PBN and lightly to the central lateral subnucleus of the PBN. These results are discussed in terms of the role of LiCl in the formation of conditioned taste aversion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers
  • Brain / cytology
  • Brain Chemistry / drug effects
  • Brain Chemistry / genetics*
  • Cerebral Ventricles / cytology
  • Cerebral Ventricles / physiology
  • Chlorides / pharmacology*
  • Gene Expression / drug effects*
  • Genes, fos / drug effects*
  • Immunohistochemistry
  • Injections, Intraperitoneal
  • Lithium / pharmacology*
  • Lithium Chloride
  • Male
  • Medulla Oblongata / cytology
  • Medulla Oblongata / physiology
  • Neurons / physiology
  • Pons / cytology
  • Pons / physiology
  • Proto-Oncogene Mas
  • Proto-Oncogene Proteins c-fos / metabolism
  • Rats
  • Rats, Wistar
  • Vagotomy


  • Biomarkers
  • Chlorides
  • MAS1 protein, human
  • Proto-Oncogene Mas
  • Proto-Oncogene Proteins c-fos
  • Lithium
  • Lithium Chloride