Mouse liver cytochrome P-450 (P-450IIIAM1): its cDNA cloning and inducibility by dexamethasone

Biochim Biophys Acta. 1992 Apr 6;1130(3):329-32. doi: 10.1016/0167-4781(92)90447-8.


A full-length cDNA complementary to mouse liver mRNA coding for one of the cytochromes P-450 (P-450) in the P-450IIIA family, namely P-450IIIM1, was isolated and completely sequenced. The sequence of this cDNA clone, pMDex13, revealed that it encoded a polypeptide of 504 deduced amino acid residues (Mr = 57,853). The deduced amino acid sequence showed 87.3 and 84.9% identity with rat P-450IIIA1 and P-450IIIA2, respectively. The NH2-terminal 24 amino acid sequences of P-450IIIAM1 were completely identical with purified mouse P-450UT protein. RNA blot analysis showed that mRNA content of hepatic P-450IIIAM1 was remarkably increased by treatment of mice with dexamethasone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • Cytochrome P-450 CYP3A
  • Cytochrome P-450 Enzyme System / chemistry
  • Cytochrome P-450 Enzyme System / genetics*
  • Dexamethasone / pharmacology*
  • Gene Expression Regulation / drug effects*
  • Liver
  • Membrane Proteins
  • Mice
  • Molecular Sequence Data
  • Sequence Homology, Nucleic Acid


  • Membrane Proteins
  • Dexamethasone
  • Cytochrome P-450 Enzyme System
  • Cyp3a11 protein, mouse
  • Cytochrome P-450 CYP3A

Associated data

  • GENBANK/M55186
  • GENBANK/M55187
  • GENBANK/M55188
  • GENBANK/M91426
  • GENBANK/M91427
  • GENBANK/M91428
  • GENBANK/M91429
  • GENBANK/M91430
  • GENBANK/X60452
  • GENBANK/X61944