CENP-C, an autoantigen in scleroderma, is a component of the human inner kinetochore plate

Cell. 1992 Jul 10;70(1):115-25. doi: 10.1016/0092-8674(92)90538-n.


We have isolated and characterized a set of overlapping cDNA clones that encode the human centromere autoantigen centromere protein C (CENP-C). The identity of these clones has been established using several criteria. First, they were shown to encode a polypeptide that migrates at the expected position for CENP-C on SDS-polyacrylamide gel electrophoresis. Second, we have demonstrated that this polypeptide shares at least two epitopes with human CENP-C. Polyclonal antibodies were raised to fusion proteins encoded by nonoverlapping regions of the cDNA clones. These antibodies were shown to recognize a protein at a position appropriate for CENP-C on immunoblots of human chromosomal proteins. In addition, we used indirect immunofluorescence to demonstrate that these antibodies recognize centromeres of HeLa chromosomes in the expected pattern for CENP-C. Localization of CENP-C by immunoelectron microscopy reveals that this protein is a component of the inner kinetochore plate.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Autoantigens / chemistry*
  • Base Sequence
  • Centromere / chemistry*
  • Chromosomal Proteins, Non-Histone / analysis*
  • Chromosomes / chemistry
  • Cloning, Molecular
  • DNA / isolation & purification
  • HeLa Cells
  • Humans
  • Molecular Sequence Data
  • Raynaud Disease / genetics*
  • Raynaud Disease / immunology


  • Autoantigens
  • Chromosomal Proteins, Non-Histone
  • centromere protein C
  • DNA

Associated data

  • GENBANK/L05499
  • GENBANK/M87278
  • GENBANK/M93428
  • GENBANK/M95724
  • GENBANK/S72766
  • GENBANK/S72767
  • GENBANK/S72768
  • GENBANK/S72769
  • GENBANK/S72771
  • GENBANK/X71664