Design of large-scale separation systems for positive and negative immunomagnetic selection of cells using superparamagnetic microspheres

J Hematother. Winter 1992;1(4):379-86. doi: 10.1089/scd.1.1992.1.379.

Abstract

The ex vivo selective separation of cells from bone marrow and peripheral blood stem cell preparations is increasingly used as an adjunct to hematopoietic rescue following high-dose therapy for refractory cancer. Immunomagnetic separation, in which the target cells are identified using monoclonal antibodies and separated by attachment to paramagnetic particles and passage through a magnetic field, is widely used for both negative and positive cell selection. In this paper, we discuss the factors that should be considered when developing a magnetic separation device for purging tumor cells and selecting stem cells from bone marrow using superparamagnetic microspheres.

Publication types

  • Review

MeSH terms

  • Animals
  • Cell Count
  • Humans
  • Immunomagnetic Separation*
  • Laboratories
  • Magnetics
  • Microspheres
  • Neoplasms / pathology
  • Neoplasms / therapy
  • Neoplasms, Experimental / pathology
  • Neoplasms, Experimental / therapy