Analysis of DNA Restriction Fragment Length Polymorphism Extends the Evidence for Breast Milk Transmission in Streptococcus Agalactiae Late-Onset Neonatal Infection

J Infect Dis. 1992 Mar;165(3):569-73. doi: 10.1093/infdis/165.3.569.

Abstract

Analysis of restriction fragment length polymorphism (RFLP) of total DNA and of ribosomal DNA (ribotyping) was used to document four cases of Streptococcus agalactiae mother-to-infant transmission potentially associated with ingestion of infected mother's milk. Twenty strains were analyzed. Ten strains were mother-baby pairs, five from the milk of five mothers, four from their neonates with late-onset infection, and one from a colonized neonate. All mothers had early postpartum mastitis. Ten unrelated strains were studied for comparison. In each case, the two strains of each mother-baby pair produced identical RFLP patterns of total DNA. The 10 unrelated strains generated 10 different patterns, one of which, though, was observed in one of the mother-baby pairs. Ribotyping was less discriminative than total DNA RFLP analysis (6 different patterns vs. 13). These data extend the evidence for breast milk transmission in S. agalactiae late-onset neonatal infection.

MeSH terms

  • Breast Feeding
  • DNA, Bacterial / analysis*
  • DNA, Ribosomal / analysis
  • Female
  • Humans
  • Infant, Newborn
  • Mastitis / microbiology
  • Milk, Human / microbiology*
  • Polymorphism, Restriction Fragment Length*
  • Pregnancy
  • Streptococcal Infections / microbiology
  • Streptococcal Infections / transmission*
  • Streptococcus agalactiae / classification
  • Streptococcus agalactiae / genetics*

Substances

  • DNA, Bacterial
  • DNA, Ribosomal