Microkinetospheres and VP satellites of pinocytic cells observed in tissue cultures of Gey's strain HeLa with phase contrast cinematographic techniques

J Biophys Biochem Cytol. 1957 Sep 25;3(5):697-704. doi: 10.1083/jcb.3.5.697.

Abstract

By tissue culture methods and with the use of phase contrast, interference color contrast, and time-lapse cinematographic equipment, the activity of a cytoplasmic organoid, termed the microkinetosphere, has been followed and correlated with pinocytosis. A transformation of several microkinetospheres by coherence and coalescence into the solitary VP satellite was observed in cells undergoing pinocytosis in serum nutrients. A correlation of both of these structures to cytoplasmic organoids described by others, notably with the electron microscope, and a hypothesis on the nature of the microkinetosphere and its transformation was presented.

MeSH terms

  • HeLa Cells*
  • Humans
  • Microscopy, Phase-Contrast*
  • Pinocytosis*
  • Serum*
  • Tissue Culture Techniques*