Immunogold labelling is a quantitative method as demonstrated by studies on aminopeptidase N in microvillar membrane vesicles

Histochem J. 1992 Mar;24(3):132-6. doi: 10.1007/BF01047462.


Microvillar membrane vesicle preparations with varying content of aminopeptidase N were prepared from enterocytes of the pig small intestine. Postembedding immunogold labelling of aminopeptidase N was performed on these glutaraldehyde/paraformaldehyde-fixed, osmium tetroxide-treated and Epon-embedded microvillar membrane vesicles. The number of gold particles per micrometre microvillar membrane (labelling intensity) was calculated and compared to the corresponding enzymatic activity. A very close relationship was found between labelling intensity and aminopeptidase N activity, demonstrating that postembedding immunogold labelling can be used quantitatively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminopeptidases / analysis*
  • Animals
  • CD13 Antigens
  • Immunohistochemistry*
  • Intestine, Small / enzymology*
  • Intestine, Small / ultrastructure
  • Microvilli / enzymology
  • Papain
  • Subcellular Fractions / enzymology
  • Swine


  • Aminopeptidases
  • CD13 Antigens
  • Papain