Binaural interaction was investigated in a 400 microns brain slice taken through the mouse lateral superior olive (LSO). Ipsilateral excitatory and contralateral inhibitory inputs to LSO neurons were examined by recording physiological responses to electrical stimulation of the trapezoid body. Bath application of non-N-methyl-D-aspartate (non-NMDA) antagonists blocked ipsilateral excitation and strychnine blocked contralateral inhibition. N-methyl-D-aspartate (NMDA) had little effect on ipsilateral responses but completely blocked contralateral inhibition. These results suggest that ipsilateral excitation is mediated by non-NMDA receptors and contralateral inhibition by strychnine dependent glycine receptors. NMDA receptors may play a role by modulating contralateral inhibition in LSO.