Human aminopeptidase N is a receptor for human coronavirus 229E

Nature. 1992 Jun 4;357(6377):420-2. doi: 10.1038/357420a0.


Human coronaviruses (HCV) in two serogroups represented by HCV-229E and HCV-OC43 are an important cause of upper respiratory tract infections. Here we report that human aminopeptidase N, a cell-surface metalloprotease on intestinal, lung and kidney epithelial cells, is a receptor for human coronavirus strain HCV-229E, but not for HCV-OC43. A monoclonal antibody, RBS, blocked HCV-229E virus infection of human lung fibroblasts, immunoprecipitated aminopeptidase N and inhibited its enzymatic activity. HCV-229E-resistant murine fibroblasts became susceptible after transfection with complementary DNA encoding human aminopeptidase N. By contrast, infection of human cells with HCV-OC43 was not inhibited by antibody RBS and expression of aminopeptidase N did not enhance HCV-OC43 replication in mouse cells. A mutant aminopeptidase lacking the catalytic site of the enzyme did not bind HCV-229E or RBS and did not render murine cells susceptible to HCV-229E infection, suggesting that the virus-binding site may lie at or near the active site of the human aminopeptidase molecule.

MeSH terms

  • Aminopeptidases / genetics
  • Aminopeptidases / immunology
  • Aminopeptidases / physiology*
  • Animals
  • Antibodies, Monoclonal
  • Binding Sites
  • CD13 Antigens
  • Cell Line
  • Cell Membrane / enzymology
  • Coronaviridae / physiology*
  • DNA / genetics
  • Fibroblasts / enzymology
  • Fibroblasts / microbiology
  • Flow Cytometry
  • Humans
  • Immunosorbent Techniques
  • Lung / enzymology
  • Lung / microbiology
  • Mice
  • Receptors, Virus / physiology*
  • Transfection


  • Antibodies, Monoclonal
  • Receptors, Virus
  • DNA
  • Aminopeptidases
  • CD13 Antigens