H-2M3 presents a Listeria monocytogenes peptide to cytotoxic T lymphocytes

Cell. 1992 Jul 24;70(2):215-23. doi: 10.1016/0092-8674(92)90097-v.

Abstract

We report evidence that a major histocompatibility complex-encoded nonclassic class I molecule presents a foreign peptide to cytotoxic T lymphocytes (CTL) during an infection. Mice immunized with virulent Listeria monocytogenes generate CD8+ CTL with alpha beta receptors specific for a bacterial peptide presented by a conserved class I molecule encoded in the M region of the major histocompatibility complex. The Listeria peptide is digested by carboxypeptidase Y but resists aminopeptidase M, and only peptides with N-formyl methionine competitively block its presentation to CTL. Transfection with the H-2M3d gene enables a negative (H-2w17) cell line to present the bacterial peptide. One function, therefore, of H-2M3 is to present bacterial peptides to CTL during infection.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Aminopeptidases / chemistry
  • Animals
  • Antigens, Bacterial / chemistry
  • Antigens, Bacterial / immunology*
  • Binding, Competitive
  • CD13 Antigens
  • CD8 Antigens / immunology
  • Carboxypeptidases / chemistry
  • Cell Line
  • Listeria monocytogenes / immunology*
  • Major Histocompatibility Complex / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • N-Formylmethionine / metabolism
  • Peptides / immunology*
  • T-Lymphocytes, Cytotoxic / cytology
  • T-Lymphocytes, Cytotoxic / immunology*

Substances

  • Antigens, Bacterial
  • CD8 Antigens
  • Peptides
  • N-Formylmethionine
  • Carboxypeptidases
  • Aminopeptidases
  • CD13 Antigens
  • serine carboxypeptidase