The apical sorting signal on human aminopeptidase N is not located in the stalk but in the catalytic head group

FEBS Lett. 1992 Aug 10;308(1):14-7. doi: 10.1016/0014-5793(92)81039-o.

Abstract

Human aminopeptidase N carries an apical sorting signal on its ectodomain necessary for its correct transport to the apical membrane in Madin-Darby canine kidney cells. To determine whether the apical sorting signal is localized in the serine/threonine rich stalk or in the catalytic head group, anchor/stalk-minus aminopeptidase N, consisting of the hemagglutinin signal peptide and the catalytic head group of human aminopeptidase N, was expressed in MDCK cells. Anchor/stalk-minus aminopeptidase N was secreted mainly to the apical side. The catalytic head group of human aminopeptidase N thus carries an apical sorting signal.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Aminopeptidases / genetics
  • Aminopeptidases / metabolism*
  • Animals
  • Blotting, Western
  • CD13 Antigens
  • Catalysis
  • Cells, Cultured
  • DNA
  • Dogs
  • Humans
  • Molecular Sequence Data
  • Precipitin Tests

Substances

  • DNA
  • Aminopeptidases
  • CD13 Antigens