Regulation of the expression of leukocyte function-associated antigen-1 (LFA-1) and intercellular adhesion molecule-1 (ICAM-1) on a human eosinophilic leukemia cell line EoL-3

Cell Immunol. 1992 Sep;143(2):335-47. doi: 10.1016/0008-8749(92)90030-s.


The effects of several cytokines and phorbol myristate acetate (PMA) on LFA-1 and ICAM-1 expression on a human eosinophilic leukemia cell line, EoL-3, were investigated and compared with those of a human monocytic leukemia cell line, U937. EoL-3 cells expressed large amounts of LFA-1 and small amounts of ICAM-1, and their expression was regulated similarly in EoL-3 cells and U937 cells. Interferon-gamma (IFN-gamma) enhanced ICAM-1 expression but not LFA-1 expression, and PMA augmented both LFA-1 and ICAM-1 expression. IFN-gamma and PMA showed an additive effect on ICAM-1 expression. These results collectively suggest that expression of LFA-1 and ICAM-1 is regulated differently and that IFN-gamma and PMA regulate the expression through different mechanisms. PMA but not IFN-gamma induced homotypic adhesion of EoL-3 and U937 cells, suggesting that PMA but not IFN-gamma activated the adhesive function of these cells. Staurosporin, an inhibitor of protein kinases (PKs), partly suppressed IFN-gamma- and PMA-augmented expression of ICAM-1 on EoL-3 and U937 cells, but did not affect PMA-augmented LFA-1 expression, suggesting that staurosporin-sensitive PKs are involved in IFN-gamma- and PMA-augmented ICAM-1 expression but not in PMA-augmented LFA-1 expression. The role of protein kinase C (PK-C) in these mechanisms was not revealed because a PK-C inhibitor, H-7, did not show any definitive effect on IFN-gamma- and PMA-induced expression of LFA-1 and ICAM-1. Moreover, cyclic AMP (cAMP)- and cGMP-dependent pathways were not shown to be involved in the augmentation of the expression of these molecules.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / metabolism
  • CD18 Antigens
  • Cell Adhesion
  • Cell Adhesion Molecules / metabolism*
  • Dexamethasone / pharmacology
  • Dose-Response Relationship, Drug
  • Eosinophils / cytology
  • Eosinophils / metabolism*
  • Humans
  • In Vitro Techniques
  • Intercellular Adhesion Molecule-1
  • Interferon-gamma / pharmacology
  • Leukemia, Eosinophilic, Acute / metabolism*
  • Leukemia, Eosinophilic, Acute / pathology
  • Lymphocyte Function-Associated Antigen-1 / metabolism*
  • Tetradecanoylphorbol Acetate / pharmacology
  • Tumor Cells, Cultured


  • Antigens, CD
  • CD18 Antigens
  • Cell Adhesion Molecules
  • Lymphocyte Function-Associated Antigen-1
  • Intercellular Adhesion Molecule-1
  • Dexamethasone
  • Interferon-gamma
  • Tetradecanoylphorbol Acetate